Universal method for producing ROX-labeled size standards suitable for automated genotyping.

Fluorescently labeled size standards are indispensable for genotyping via an automated DNA sequencer. Microsatellites, amplified fragment-length polymorphisms (AFLPs), single nucleotide polymorphisms (SNPs), and random amplified polymorphic DNA (RAPD) can all be assayed on platforms such as the ABI Prism® 377 DNA Sequencer or the ABI Prism 3100-Avant Genetic Analyzer (both from Applied Biosystems, Foster City, CA, USA), but each requires a fluorescent marker that typically is purchased from a commercial vendor. These markers are often prohibitively expensive. Indeed, the cost of size standards can exceed that of the genotyping assay itself. In response to Universal method for producing ROX-labeled size standards suitable for automated genotyping J. Andrew DeWoody1, Jim Schupp2, Leo Kenefic2, Joseph Busch2, Lisa Murfitt1, and Paul Keim2 1Purdue University, West Lafayette, IN and 2Northern Arizona University, Flagstaff, AZ, USA